Technician

Job Purpose

To provide in-depth technical expertise for creating inducible gene knockouts in human malaria parasites (Plasmodium falciparum) and provide advice on the set up and best operation of equipment and procedures to aid delivery objectives. This post is based within the School of Infection and Immunity, working under the supervision of Dr Abhinay Ramaprasad (Principal Investigator) whose main research focus is to study genes that control parasite replication within red blood cells, a critical process that enables malaria parasites to multiply and progress through the clinically significant blood-stage infection. The postholder will use state-of-the-art gene-editing technologies such as CRISPR-Cas+ and DiCre to inducibly knock out essential genes in the parasite.
Main Duties and Responsibilities

Provide in-depth technical support for generation of inducible gene knockout mutant parasite lines. This involves developing and implementing streamlined molecular cloning protocols to produce transfection-ready plasmids at scale.
Provide assistance and technical support relating to malaria culture work in a Containment Level 3 laboratory. This involves day-to-day maintenance of P. falciparum cultures and transfections to produce genetically modified parasite lines.
In liaison with PI to maintain the group's laboratory equipment and diagnose faults and troubleshoot day-to-day problems relating to equipment, techniques, procedures, and protocols.
In liaison with the PI, advise, implement, and evaluate improvements to existing protocols relevant as required.
Collect, analyse and interpret data using standard procedures and ensure that accurate research records are kept, and databases are updated accordingly.
Plan and organise day-to-day work to ensure that the experimental goals are delivered within previously agreed timescales.
To supervise junior staff, where appropriate, and instruct, train and provide guidance to research staff and students in the techniques and operation of equipment and apparatus as directed by the supervisor, lab manager or PI.
Contribute to the well organised functioning of the laboratory and assist the PI/laboratory manager in maintaining the stock levels and purchasing consumables and supplies, within budget, and in consultation with other members of the group.
In consultation with the PI/laboratory manager, ensure the safe and efficient functioning of the laboratory area and facilities. This includes, but is not limited to, supporting the completion of risk assessments and COSHH assessments, ensuring that all Health and Safety paperwork is completed timeously and recorded accurately, and implementing and adhering to health and safety policies, as determined by the University and the Health and Safety Executive.
Collaborate with colleagues and participate in team meetings/discussions and departmental research group activities.
Keep up to date with advancements in technology and methodology, continually updating technical knowledge.
Be aware of and comply with Equality and Diversity policies of the University of Glasgow.
Knowledge/Qualifications, Skills and Experience

Knowledge/Qualifications

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